3 . measuring expression of the associated molecular chaperone grp78 and the enzyme protein disulphide isomerase pdi affected by low expression of grp94 . ( 1 ) detecting mrna and p 检测grp94表达水平下降对相关分子伴侣grp78和pdi的影响( 1 )分别通过rt - pcr方法和westernblot方法从mrna和蛋白水平检测分子伴侣grp78的表达情况。